Recombinant Human OGG1 protein, His-tagged

• Cat.No.: OGG1-29565TH
• Product Overview: Recombinant Human OGG1(1-345 aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: OGG1, also known as 8-oxoguanine glycosylase, is a DNA glycosylase enzyme involved in base excision repair. This protein is the primary enzyme responsible for the excision of 7,8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen species (ROS). It has a beta lyase activity that nicks DNA 3' to the lesion.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) Containing 100 mM NaCl, 40% Glycerol
• Molecular Mass: 41.2 kDa(368aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH TGSMPARALL PRRMGHRTLA STPALWASIP CPRSELRLDL VLPSGQSFRW REQSPAHWSG VLADQVWTLTQTEEQLHCTV YRGDKSQASR PTPDELEAVR KYFQLDVTLA QLYHHWGSVD SHFQEVAQKF QGVRLLRQDP IECLFSFICS SNNNIARITGMVERLCQAFG PRLIQLDDVT YHGFPSLQAL AGPEVEAHLR KLGLGYRARY VSASARAILE EQGGLAWLQQ LRESSYEEAH KALCILPGVGTKVADCICLM ALDKPQAVPV DVHMWHIAQRDYSWHPTTSQ AKGPSPQTNK ELGNFFRSLW GPYAGWAQAV LFSADLRQCR HAQEPPAKRRKGSKGPEG
• Purity: > 90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: OGG1 8-oxoguanine DNA glycosylase [ Homo sapiens ]
• Official Symbol: OGG1
• Synonyms: OGG1; 8-oxoguanine DNA glycosylase; N-glycosylase/DNA lyase; 8 hydroxyguanine DNA glycosylase; HMMH; HOGG1; MUTM; OGG1 type 1d; OGG1 type 1e; OGG1 type 1g; OGG1 type 1h; OGH1; AP lyase; OGG1 type 1f; 8-hydroxyguanine DNA glycosylase; DNA-apurinic or apyrimidinic site lyase
• Gene ID: 4968
• mRNA Refseq: NM_002542
• Protein Refseq: NP_002533
• MIM: 601982
• UniProt ID: O15527
• Chromosome Location: 3p26
• Pathway: Base Excision Repair, organism-specific biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Base-Excision Repair, AP Site Formation, organism-specific biosystem; Base-free sugar-phosphate removal via the single-nucleotide replacement pathway, organism-specific biosystem
• Function: damaged DNA binding; endonuclease activity; hydrolase activity, acting on glycosyl bonds; lyase activity; oxidized purine base lesion DNA N-glycosylase activity; protein binding