Recombinant Human OAT protein, His & T7-tagged

• Cat.No.: OAT-1039H
• Product Overview: Recombinant Human OAT aa. (Met1~Phe301 (Accession # P04181) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Met1~Phe301
• Description: This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 36.6kDa.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.2.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: OAT ornithine aminotransferase [ Homo sapiens (human) ]
• Official Symbol: OAT
• Synonyms: OAT; ornithine aminotransferase; OKT; GACR; HOGA; OATASE; ornithine aminotransferase, mitochondrial; gyrate atrophy; ornithine delta-aminotransferase; ornithine-oxo-acid aminotransferase; testicular tissue protein Li 130
• Gene ID: 4942
• mRNA Refseq: NM_000274.3
• Protein Refseq: NP_000265.1
• UniProt ID: P04181