Recombinant Human NUP188 protein, His & GST-tagged

• Cat.No.: NUP188-5780H
• Product Overview: Recombinant Human NUP188 aa. (Thr540~Gly782 (Accession # Q5SRE5) ) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: The nuclear pore complex (NPC) is found on the nuclear envelope and forms a gateway that regulates the flow of proteins and RNAs between the cytoplasm and nucleoplasm. The NPC is comprised of approximately 30 distinct proteins collectively known as nucleoporins. Nucleoporins are pore-complex-specific glycoproteins which often have cytoplasmically oriented O-linked N-acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. However, the nucleoporin protein encoded by this gene does not contain the typical FG repeat sequences found in most vertebrate nucleoporins. This nucleoporin is thought to form part of the scaffold for the central channel of the nuclear pore.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: 55kDa as determined by SDS-PAGE reducing conditions
• Protein length: Thr540~Gly782
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.4.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: NUP188 nucleoporin 188 [ Homo sapiens (human) ]
• Official Symbol: NUP188
• Synonyms: hNup188; KIAA0169; nucleoporin 188kDa; nucleoporin NUP188 homolog
• Gene ID: 23511
• mRNA Refseq: NM_015354.2
• Protein Refseq: NP_056169.1
• UniProt ID: Q5SRE5