Recombinant Human NUP133 protein, His & T7-tagged

• Cat.No.: NUP133-1542H
• Product Overview: Recombinant Human NUP133 aa. (Asp899~Asp1091 (Accession # Q8WUM0)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.2kDa.
• Protein length: Asp899~Asp1091
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: >95%
• Characteristic: The isoelectric point is 5.5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: NUP133 nucleoporin 133 [ Homo sapiens (human) ]
• Official Symbol: NUP133
• Synonyms: hNUP133; 133 kDa nucleoporin; nucleoporin 133kD; nucleoporin 133kDa; nucleoporin Nup133; nuclear pore complex protein Nup133
• Gene ID: 55746
• mRNA Refseq: NM_018230.2
• Protein Refseq: NP_060700.2
• UniProt ID: Q8WUM0