Recombinant Human Nucleosome, H3.3K36me3dNuc, Biotinylated

• Cat.No.: NUC-028
• Product Overview: Mononucleosomes assembled from recombinant human histones expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.3-P84243; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary synthetic method) contains trimethylated lysine at position 36. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers, is useful for nucleosome assembly, and contains a 5’ biotin-TEG group.
• Form: Nucleosome, Recombinant Human, H3.3K36me3 (27.4 μg protein weight, 50 μg DNA+protein) in 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol.
• Molecular Mass: 199.749.4 Da
• Applications: Nucleosome, Recombinant Human, H3.3K36me3 dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. Nucleosome, Recombinant Human, H3.3K36me3 dNucs does not contain free DNA which could alter assayed activities.
• Storage: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.