Recombinant Human NSL1, MIS12 kinetochore complex component, His-tagged

• Cat.No.: NSL1-260H
• Product Overview: NSL1, 1-281aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-281 a.a.
• Description: NSL1 is a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human NSL1 protein, fused to His-tag at N-terminus, was expressed in E.coli .
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol 0.4M Urea
• Molecular Mass: 34.6kDa (304aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGSPEL VVLDPPWDKE LAAGTESQAL VSATPREDFR VRCTSKRAVT EMLQLCGRFV QKLGDALPEE IREPALRDAQ WTFESAVQEN ISINGQAWQE ASDNCFMDSD IKVLEDQFDE IIVDIATKRK QYPRKILECV IKTIKAKQEI LKQYHPVVHP LDLKYDPDPA PHMENLKCRG ETVAKEISEA MKSLPALIEQ GEGFSQVLRM QPVIHLQRIH QEVFSSCHRK PDAKPENFIT QIETTPTETA SRKTSDMVLK RKQTKDCPQR KWYPLRPKKI NLDT
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: NSL1 NSL1, MIND kinetochore complex component, homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: NSL1
• Synonyms: NSL1; NSL1, MIND kinetochore complex component, homolog (S. cerevisiae); C1orf48, chromosome 1 open reading frame 48; kinetochore-associated protein NSL1 homolog; DC8; DKFZP566O1646; MIS14; C1orf48; DKFZp566O1646
• Gene ID: 25936
• mRNA Refseq: NM_001042549
• Protein Refseq: NP_001036014
• MIM: 609174
• UniProt ID: Q96IY1
• Chromosome Location: 1q41
• Pathway: Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; DNA Replication, organism-specific biosystem; M Phase, organism-specific biosystem; Mitotic M-M/G1 phases, organism-specific biosystem; Mitotic Prometaphase, organism-specific biosystem
• Function: protein binding