Recombinant Human NRG4 Protein

• Cat.No.: NRG4-09H
• Product Overview: Recombinant human Neuregulin-4 EGF domain is a disulfide-linked monomeric protein consisting of 62 amino acid residue subunits, and migrates as an approximately 7 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Neuregulin-4 EGF domain was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 5–65 aa
• Form: Lyophilized protein
• Storage: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20 centigrade. Upon reconstitution, store in working aliquots at +4 centigrade for up to one month, or at -20 centigrade for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered 20 mM phosphate buffer, pH 6.0.
• Bio-activity: The ED50 was determined by the phosphorylation of ErbB2 and ErbB4 receptors in CHO cells, and was found to be < 3 ng/mL, corresponding to a specific activity of 3×10^5 Units/mg.
• Endotoxin: < 0.1 ng/μg (1 EU/μg), using the LAL gel clot method.
• Purity: ≥ 96% by SDS-PAGE and HPLC
• Shipping: Gel packs or dry ice

Gene Information

• Official Symbol: NRG4
• Synonyms: NRG4; neuregulin 4; HRG4; pro-neuregulin-4, membrane-bound isoform; heregulin 4; pro-NRG4
• Gene ID: 145957
• mRNA Refseq: NM_138573
• Protein Refseq: NP_612640
• MIM: 610894
• UniProt ID: Q8WWG1