Recombinant Human NR5A2 protein, His-tagged

• Cat.No.: NR5A2-1092H
• Product Overview: Recombinant Human NR5A2 aa. (Ser300~Ala541 fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a DNA-binding zinc finger transcription factor and is a member of the fushi tarazu factor-1 subfamily of orphan nuclear receptors. The encoded protein is involved in the expression of genes for hepatitis B virus and cholesterol biosynthesis, and may be an important regulator of embryonic development.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 29kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ser300~Ala541
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.7.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: NR5A2 nuclear receptor subfamily 5 group A member 2 [ Homo sapiens (human) ]
• Official Symbol: NR5A2
• Synonyms: NR5A2; nuclear receptor subfamily 5 group A member 2; B1F; CPF; FTF; B1F2; LRH1; LRH-1; FTZ-F1; hB1F-2; FTZ-F1beta; nuclear receptor subfamily 5 group A member 2; CYP7A promoter-binding factor; b1-binding factor, hepatocyte transcription factor which activates enhancer II of hepatitis B virus; fetoprotein-alpha 1 (AFP) transcription factor; hepatocytic transcription factor hB1F-3; liver nuclear receptor homolog-1 variant 2
• Gene ID: 2494
• mRNA Refseq: NM_001276464.1
• Protein Refseq: NP_001263393.1
• UniProt ID: O00482