Recombinant Human NR3C2 Protein, His/GST-tagged

• Cat.No.: NR3C2-693H
• Product Overview: Recombinant Human NR3C2(Val739~Lys984) fused with His/GST tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Val739~Lys984
• Description: This gene encodes the mineralocorticoid receptor, which mediates aldosterone actions on salt and water balance within restricted target cells. The protein functions as a ligand-dependent transcription factor that binds to mineralocorticoid response elements in order to transactivate target genes. Mutations in this gene cause autosomal dominant pseudohypoaldosteronism type I, a disorder characterized by urinary salt wasting. Defects in this gene are also associated with early onset hypertension with severe exacerbation in pregnancy. Alternative splicing results in multiple transcript variants.
• Form: 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: 58.71kDa
• Identity: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: NR3C2 nuclear receptor subfamily 3, group C, member 2 [ Homo sapiens ]
• Official Symbol: NR3C2
• Synonyms: NR3C2; nuclear receptor subfamily 3, group C, member 2; MLR; mineralocorticoid receptor; MR; aldosterone receptor; mineralocorticoid receptor 1; mineralocorticoid receptor delta; MCR; NR3C2VIT; FLJ41052; MGC133092
• Gene ID: 4306
• mRNA Refseq: NM_000901
• Protein Refseq: NP_000892
• MIM: 600983
• UniProt ID: P08235