Recombinant Human NR3C1 protein, His-tagged

• Cat.No.: NR3C1-1044H
• Product Overview: Recombinant Human NR3C1 fused with His tag was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Form: The protein is in 20mM Tris-HCl pH7.9, 300mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol.
• Molecular Mass: 87.5 kDa.
• AA Sequence: MDSKESLTPG REENPSSVLA QERGDVMDFY KTLRGGATVK VSASSPSLAV ASQSDSKQRRLLVDFPKGSV SNAQQPDLSK AVSLSMGLYM GETETKVMGN DLGFPQQGQI SLSSGETDLKLLEESIANLN RSTSVPENPK SSASTAVSAA PTEKEFPKTH SDVSSEQQHL KGQTGTNGGNVKLYTTDQST FDILQDLEFS SGSPGKETNE SPWRSDLLID ENCLLSPLAG EDDSFLLEGNSNEDCKPLIL PDTKPKIKDN GDLVLSSPSN VTLPQVKTEK EDFIELCTPG VIKQEKLGTVYCQASFPGAN IIGNKMSAIS VHGVSTSGGQ MYHYDMNTAS LSQQQDQKPI FNVIPPIPVGSENWNRCQGS GDDNLTSLGT LNFPGRTVFS NGYSSPSMRP DVSSPPSSSS TATTGPPPKLCLVCSDEASG CHYGVLTCGS CKVFFKRAVE GQHNYLCAGR NDCIIDKIRR KNCPACRYRKCLQAGMNLEA RKTKKKIKGI QQATTGVSQE TSENPGNKTI VPATLPQLTP TLVSLLEVIEPEVLYAGYDS SVPDSTWRIM TTLNMLGGRQ VIAAVKWAKA IPGFRNLHLD DQMTLLQYSWMFLMAFALGW RSYRQSSANL LCFAPDLIIN EQRMTLPCMY DQCKHMLYVS SELHRLQVSYEEYLCMKTLL LLSSVPKDGL KSQELFDEIR MTYIKELGKA IVKREGNSSQ NWQRFYQLTKLLDSMHEVVE NLLNYCFQTF LDKTMSIEFP EMLAEIITNQ IPKYSNGNIK KLLFHQK
• Purity: greater than 90% based on SDS-PAGE analysis.
• Unit Definition: 1 unit equals 1 nanogram of purified protein. 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interactions assay.
• Storage: Stored at -70 centigrade before use. Avoid repeated freeze thaw cycles.

Gene Information

• Gene Name: NR3C1 nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) [ Homo sapiens ]
• Official Symbol: NR3C1
• Synonyms: NR3C1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); GRL, nuclear receptor subfamily 3, group C, member 1; glucocorticoid receptor; GR; glucocorticoid nuclear receptor variant 1; GCR; GRL; GCCR
• Gene ID: 2908
• mRNA Refseq: NM_000176
• Protein Refseq: NP_000167
• UniProt ID: P04150
• Chromosome Location: 5q31-q32
• Pathway: Adipogenesis, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Gene expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem;
• Function: Hsp70 protein binding; Hsp90 protein binding; chromatin binding; double-stranded DNA binding; glucocorticoid receptor activity; metal ion binding; protein binding; protein heterodimerization activity; protein homodimerization activity; receptor activity; receptor tyrosine kinase binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; steroid binding; zinc ion binding