Recombinant Human NPPA protein, His & GST-tagged

• Cat.No.: NPPA-2689H
• Product Overview: Recombinant Human NPPA aa. (Ala25~Arg123) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1.
• Source: E. coli
• Species: Human
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: 41kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ala25~Arg123
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.7.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: NPPA natriuretic peptide A [ Homo sapiens (human) ]
• Official Symbol: NPPA
• Synonyms: ANF; ANP; CDD; CDP; PND; ATFB6; ATRST2; CDD-ANF; atriopeptin; cardiodilatin-related peptide; cardionatrin; natriuretic peptide precursor A variant 1; prepronatriodilatin; natriuretic peptides A
• Gene ID: 4878
• mRNA Refseq: NM_006172.3
• Protein Refseq: NP_006163.1
• UniProt ID: P01160