Recombinant Human Noggin

• Cat.No.: NOG-454H
• Product Overview: Recombinat human Noggin was expressed in modifiedhuman 293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Noggin is a secreted glycoprotein predominantly expressed by the dorsal mesoderm during embryogenesis. Noggin has been shown to bind with high affinity to bone morphogenetic protein-4 (BMP-4) and with lower affinity to BMP-2 and BMP-7. Noggin specifically inhibits the action of BMP's by preventing interaction with their receptors. It has been shown that noggin maintains hES in an undifferentiated state in culture. Additionally, noggin in combination with FGF2 can maintain undifferentiated hES growth for substantial culture periods while retaining hES karyotype and pluripotency. The role of noggin in the maintenance of undifferentiated hES suggests it is potentially a valuable component of animal free hES cultures allowing hES to be applicable for therapeutic applications. Human noggin is a 205 amino acid glycoprotein and is secreted as a covalently linked homodimer of approximately 30 kDa with a similar structure to BMP-7.
• Amino Acid Sequence: QHYLHIRPAPS DNLPLVDLIEHPDPIFDPK EKDLNETLLRSLLGGHYD PGFM ATSP P ED RPG GGGGA AGG AEDLAELDQLLRQRP SGA MPS EIKGLE FSEGLA Q GKKQRLSKKLRRKLQMWL WSQTFCPVLYAWNDLGS RFWPR YVK V GSCFSKRS CSVPEGMVCKPSKSVHL TVL RWR CQRR GGQ RCGW IPIQY PIISECKCSC.
• Molecular Mass: Noggin migrates as a band between 25 and 30 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified Noggin that has a predicted molecular mass of 23.0kDa.
• PI: Noggin separates into a number of isoforms with a pI between 4.2 and 5.3 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified Noggin that has a predicted pI of 9.1.
• % Carbohydrate: Noggin consists of 5-25% carbohydrate by weight.
• Glycosylation: Noggin has N-linked oligosaccharides.
• Purity: >95% as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C.

Gene Information

• Gene Name: NOG noggin [ Homo sapiens ]
• Synonyms: noggin; SYM1; SYNS1; NOG; symphalangism 1 (proximal); synostoses (multiple) syndrome 1
• Gene ID: 9241
• mRNA Refseq: NM_005450
• Protein Refseq: NP_005441
• MIM: 602991
• UniProt ID: Q13253
• Chromosome Location: 17q21-q22
• Pathway: TGF-beta signaling pathway; Signaling by BMP
• Function: protein homodimerization activity; cytokine binding