Recombinant Human NME4, His-tagged

• Cat.No.: NME4-795H
• Product Overview: Recombinant human NME4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (33-187aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: NME4, also known as nucleoside diphosphate kinase, mitochondrial, belongs to the NDK family. NME4 are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4. NME4 plays a major role in the synthesis of nucleoside triphosphates other than ATP.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.2M NaCl
• Molecular Weight: 19.6 kDa (176aa) confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYMSSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: NME4 non-metastatic cells 4, protein expressed in[ Homo sapiens ]
• Synonyms: NME4; NDPK-D; NM23H4; nm23-H4; nucleoside diphosphate kinase, mitochondrial; NDK; nucleoside diphosphate kinase D; NDPKD;NDP kinase D; OTTHUMP00000194917; OTTHUMP00000194919; OTTHUMP00000206530; NDP kinase, mitochondrial; NP_005000.1; EC 2.7.4.6
• Gene ID: 4833
• mRNA Refseq: NM_005009
• Protein Refseq: NP_005000
• MIM: 601818
• UniProt ID: O00746
• Chromosome Location: 16p13.3
• Function: ATP binding; kinase activity; metal ion binding; nucleoside diphosphate kinase activity; nucleotide binding; transferase activity