Recombinant Human NISCH Protein, His/GST-tagged

• Cat.No.: NISCH-299H
• Product Overview: Recombinant Human NISCH(Leu1246~Ser1346) fused with His/GST tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Leu1246~Ser1346
• Description: This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor.
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 43.8kDa
• Identity: Reconstitute in PBS or others
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: NISCH nischarin [ Homo sapiens ]
• Official Symbol: NISCH
• Synonyms: NISCH; nischarin; I 1; I 1 receptor candidate protein; imidazoline receptor antisera selected; imidazoline receptor candidate; IRAS; KIAA0975; IR1; hIRAS; I1R candidate protein; imidazoline receptor 1; I-1 receptor candidate protein; imidazoline-1 receptor candidate protein; imidazoline receptor antisera-selected protein; I-1; FLJ14425; FLJ40413; FLJ90519
• Gene ID: 11188
• mRNA Refseq: NM_007184
• Protein Refseq: NP_009115
• UniProt ID: Q9Y2I1