Recombinant Human NIN protein, His & T7-tagged

• Cat.No.: NIN-7983H
• Product Overview: Recombinant Human NIN aa. (Met1~Glu153 (Accession # Q8N4C6)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Met1~Glu153
• Description: This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 21.3kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 4.5.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: NIN ninein [ Homo sapiens (human) ]
• Official Symbol: NIN
• Synonyms: SCKL7; glycogen synthase kinase 3 beta-interacting protein; hNinein; ninein (GSK3B interacting protein); ninein centrosomal protein; ninein
• Gene ID: 51199
• mRNA Refseq: NM_016350.4
• Protein Refseq: NP_057434.4
• UniProt ID: Q8N4C6