Recombinant Human NFYB Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: NFYB-224H
• Product Overview: NFYB MS Standard C13 and N15-labeled recombinant protein (NP_006157) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]
• Molecular Mass: 22.8 kDa
• AA Sequence: MTMDGDSSTTDASQLGISADYIGGSHYVIQPHDDTEDSMNDHEDTNGSKESFREQDIYLPIANVARIMKNAIPQTGKIAKDAKECVQECVSEFISFITSEASERCHQEKRKTINGEDILFAMSTLGFDSYVEPLKLYLQKFREAMKGEKGIGGAVTATDGLSEELTEEAFTNQLPAGLITTDGQQQNVMVYTTSYQQISGVQQIQFSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NFYB nuclear transcription factor Y, beta [ Homo sapiens (human) ]
• Official Symbol: NFYB
• Synonyms: NFYB; nuclear transcription factor Y, beta; nuclear transcription factor Y subunit beta; CBF A; HAP3; NF YB; Transcription factor NF-Y, B subunit; CAAT box DNA-binding protein subunit B; nuclear transcription factor Y subunit B; CCAAT-binding transcription factor subunit A; CBF-A; CBF-B; NF-YB
• Gene ID: 4801
• mRNA Refseq: NM_006166
• Protein Refseq: NP_006157
• MIM: 189904
• UniProt ID: P25208