Recombinant Human NFIC 293 Cell Lysate

• Cat.No.: NFIC-3851HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for nuclear factor I/C (CCAAT-binding transcription factor) (NFIC), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: NFIC nuclear factor I/C (CCAAT-binding transcription factor) [ Homo sapiens ]
• Official Symbol: NFIC
• Synonyms: NFIC; nuclear factor I/C (CCAAT-binding transcription factor); NFI; nuclear factor 1 C-type; CTF; CTF5; NF I; NF1-C; NF-I/C; TGGCA-binding protein; CCAAT-box-binding transcription factor; NF-I; MGC20153
• Gene ID: 4782
• mRNA Refseq: NM_001245002
• Protein Refseq: NP_001231931
• MIM: 600729
• UniProt ID: P08651
• Chromosome Location: 19p13.3
• Pathway: FOXA1 transcription factor network, organism-specific biosystem; Gene Expression, organism-specific biosystem; RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription, organism-specific biosystem; RNA Polymerase III Abortive And Retractive Initiation, organism-specific biosystem; RNA Polymerase III Transcription, organism-specific biosystem; RNA Polymerase III Transcription Termination, organism-specific biosystem
• Function: DNA binding; sequence-specific DNA binding transcription factor activity