Recombinant Human NEWENTRY, 777-907aa, Fc Chimera

• Cat.No.: NEWENTRY-718H
• Product Overview: Recombinant Human NEWENTRY encoding the extracellular domain (Met 1- Lys 700) linked with the cytoplasmic domain (Arg 777- Val 907) of human CMV gB (AAA45920.1) precursor was fused with the Fc region of human IgG1 at the C-terminus. It was produced inHuman Cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-700 a.a.
• Description: Human cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B is a 907-amino acid glycoprotein encoded by the ORF of UL55. It is the most abundant component of the envelope with at least two defined neutralizing epitopes, a target of neutralizing antibodies and an essential replication component. The synthesized gB precursor matures into a 130-160 kDa glycoprotein by acquiring N-linked glycosylation modifications, and is cleaved by the cellular protease furin into two components, a 93-116 kDa N-terminal fragment and a 55 kDa C-terminal fragment linked by a disulfide-bond, which is presented on the viral envelope as well as on the surface of virus-infected cells as a covalently associated homodimer. gB plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. It triggers penetration of cells and enhances the spread of infection in nonpolarized human fibroblasts. The interaction of gB with the non-heparin receptor CD209/DC-SIGN is the initiation of intracellular signaling and activation of the IFN-responsive pathway.
• Molecular Mass: The recombinant glycoprotein B of human CMV /Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 1045 amino acids and has a predicted molecular mass of 118 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh gB/Fc monomer is approximately 150-160 kDa due to glycosylation.
• Predicted N Terminal: Ser 25.
• Purity: > 90%, as determined by SDS-PAGE.
• Formulation: Supplied as a 0.2μm filtered solution of 100mM Gly, 10mM NaCl, 50 mMTris, pH 7.5.
• Stability: Samples are stable for up to twelve months from date of receipt -70℃.
• Endotoxin: < 1.0 EU per 1μg cytokine as determined by the LAL method.
• Reconstitution: Follow the instructions on the vial. Centrifuge the vial at 4 before opening to recover the entire contents.
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: NEWENTRY Record to support submission of GeneRIFs for a gene not in Entrez Gene (Human cytomegalovirus; Human cytomegalovirus HCMV; human cytomegalovirus CMV; human herpesvirus type 5). [ Human herpesvirus 5 ]
• Synonyms: NEWENTRY; Record to support submission of GeneRIFs for a gene not in Entrez Gene (Human cytomegalovirus; Human cytomegalovirus HCMV; human cytomegalovirus CMV; human herpesvirus type 5)
• Gene ID: 5495302