Recombinant Human Nei Endonuclease VIII-Like 1 (E. coli), His-tagged

• Cat.No.: NEIL1-4827H
• Product Overview: Recombinant human NEIL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF.
• Molecular Weight: 45.8 kDa (410aa), confirmed by MALDI-TOF.
• Purity: > 90% by SDS-PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MPEGPELHLA SQFVNEACRA LVFGGCVEKS SVSRNPEVPF ESSAYRISAS ARGKELRLIL SPLPGAQPQQEPLALVFRFG MSGSFQLVPR EELPRHAHLR FYTAPPGPRL ALCFVDIRRF GRWDLGGKWQ PGRGPCVLQE YQQFRENVLR NLADKAFDRPICEALLDQRF FNGIGNYLRA EILYRLKIPP FEKARSVLEA LQQHRPSPEL TLSQKIRTKL QNPDLLELCH SVPKEVVQLG GKGYGSESGEEDFAAFRAWL RCYGMPGMSS LQDRHGRTIW FQGDPGPLAP KGRKSRKKKS KATQLSPEDR VEDALPPSKA PSRTRRAKRD LPKRTATQRPEGTSLQQDPE APTVPKKGRR KGRQAASGHC RPRKVKADIP SLEPEGTSAS
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Base excision repair

Gene Information

• Gene Name: NEIL1 nei endonuclease VIII-like 1 (E. coli) [ Homo sapiens ]
• Official Symbol: NEIL1
• Synonyms: NEIL1; nei endonuclease VIII-like 1 (E. coli); FPG1; NEI1; hFPG1; FLJ22402; endonuclease 8-like 1; nei homolog 1; endonuclease VIII; OTTHUMP00000183482; nei-like protein 1; endonuclease VIII-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; DNA glycosylase/AP lyase Neil1; Endonuclease VIII-like 1; EC 3.2.2; Nei homolog 1; EC 4.2.99.18; Nei-like protein 1
• Gene ID: 79661
• mRNA Refseq: NM_024608
• Protein Refseq: NP_078884
• MIM: 608844
• UniProt ID: Q96FI4
• Chromosome Location: 15q24.2
• Function: DNA N-glycosylase activity; DNA-(apurinic or apyrimidinic site) lyase activity; damaged DNA binding; hydrolase activity, acting on glycosyl bonds; lyase activity; protein C-terminus binding; zinc ion binding