Recombinant Human NEFH protein, His/SUMO-tagged

• Cat.No.: NEFH-1255H
• Product Overview: Recombinant Human NEFH (Lys671~Lys1020) protein was fused to His/SUMO-tag at the N-terminus and expressed in E.coli.

Specification

• Description: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.
• Source: E.coli
• Species: Human
• Tag: His&SUMO
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: Predicted Molecular Mass: 52.3 kDa; Observed Molecular Mass: 54 kDa.
• Protein length: Lys671-Lys1020
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: NEFH
• Official Symbol: NEFH
• Synonyms: NFH; CMT2CC
• Gene ID: 4744
• mRNA Refseq: NM_021076.4
• Protein Refseq: NP_066554.2
• MIM: 162230
• UniProt ID: P12036