Native Bovine NEFH Protein

• Cat.No.: NEFH-181B
• Product Overview: Native Bovine NEFH Protein

Specification

• Description: Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, d are composed predominantly of four major proteins called NF-L, NF-M, NF-H and α-internexin. NF-H is the neurofilament heavy or high molecular weight polypeptide and runs on SDS-PAGE gels at 200-220kDa in the heavily phosphorylated axonal form. The molecule has an unusual and interesting region consisting of multiple Lysine-Serine-Proline peptides, about 40 of these in human. These peptide repeats are heavily phosphorylated on the Serine residues in axons. Enzymatic removal of these phosphate groups will increase the SDS-PAGE mobility to about 160kDa, likely due to protein conformational changes due to the removal of charge. Even the non-phosphorylated form runs aberrantly on SDS-PAGE, as the real molecular weight of NF-H is about 110kDa, with some variation in different species. This is likely due to an unusually high content of charged amino acids. Non-phosphorylated forms of NF-H are found in dendrites and perikarya and early in development, but the majority of NF-H in the adult is this heavily phosphorylated axonal form. Our preparation was isolated from cow spinal cord using a modification of the method of Leung and Liem, which purifies out the heavily phosphorylated axonal form, often referred to as pNF-H. The HGNC name for this protein is NEFH.
• Species: Bovine
• Molecular Mass: ~220kDa by SDS-PAGE
• Applications: ELISA, western blotting standard
• Storage: Store at -20 centigrade
• Concentration: 1 mg/mL
• Storage Buffer: 6M Urea

Gene Information

• Gene Name: NEFH neurofilament heavy chain [ Bos taurus (cattle) ]
• Official Symbol: NEFH
• Synonyms: NEFH; neurofilament heavy chain; NF-H; LOW QUALITY PROTEIN: neurofilament heavy polypeptide; 200 kDa neurofilament protein; Neurofilament triplet H protein; neurofilament heavy polypeptide
• Gene ID: 528842
• mRNA Refseq: XM_005218112
• Protein Refseq: XP_005218169
• UniProt ID: F1MSQ6