Recombinant Human NEDD4, FLAG-tagged

• Cat.No.: NEDD4-29278TH
• Product Overview: Recombinant fragment corresponding to amino acids 150-900 of Human NEDD4 Isoform 4 with N terminal FLAG tag, MWt 86kDa.

Specification

• Description: E3 ubiquitin-protein ligase NEDD4 is an enzyme that in humans is encoded by the NEDD4 gene.
• Protein length: 751 amino acids
• Conjugation: FLAG
• Molecular Weight: 86.000kDa inclusive of tags
• Source: Baculovirus
• Biological activity: Specific Activity: 0.0027 pmol/min/μg.Assay conditions: 50 μl reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5 μg), E2 (2.8 μg), Biotin-Ubiquitin (0.5 μg) and NEDD4 (0-2 μg) in 50mM Tris pH 7.4) incubate for 30min at 37°C, then add to streptavidin coate
• Form: Liquid
• Storage buffer: pH: 8.00Constituents:20% Glycerol, 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Tween, 0.0462% DTTNote: 100 μg/ml FLAG peptide
• Storage: Store at -80°C
• Sequence Similarities: Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.Contains 4 WW domains.

Gene Information

• Gene Name: NEDD4 neural precursor cell expressed, developmentally down-regulated 4 [ Homo sapiens ]
• Official Symbol: NEDD4
• Synonyms: NEDD4; neural precursor cell expressed, developmentally down-regulated 4; E3 ubiquitin-protein ligase NEDD4; KIAA0093; MGC176705; NEDD4 1; receptor potentiating factor 1; RPF1
• Gene ID: 4734
• mRNA Refseq: NM_006154
• Protein Refseq: NP_006145
• MIM: 602278
• Uniprot ID: P46934
• Chromosome Location: 15q
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem
• Function: RNA polymerase binding; acid-amino acid ligase activity; beta-2 adrenergic receptor binding; ligase activity; phosphoserine binding