Recombinant Human NECTIN1 protein, His-Avi-tagged, Biotinylated

Cat.No. : NECTIN1-188H
Product Overview : Biotinylated Recombinant Human NECTIN1 protein(Q15223-1)(Gln31-Thr334), fused with C-terminal His and Avi tag, was expressed in HEK293.
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Source : HEK293
Species : Human
Tag : C-His-Avi
Protein length : Gln31-Thr334
Conjugation/Label : Biotin
Form : Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human Nectin-3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Nectin-1, His Tag with the EC50 of 0.52ug/ml determined by ELISA (QC Test).
Molecular Mass : The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.
Endotoxin : Less than 1EU per μg by the LAL method.
Purity : > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Storage : Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Reconstitution : Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Not For Human Consumption!

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