Recombinant Human NECAP2, His-tagged

• Cat.No.: NECAP2-3483H
• Product Overview: Recombinant human NECAP2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: NECAP2, also known as adaptin ear-binding coat-associated protein 2, is a essential protein paralogues for clathrin-mediated membrane trafficking that are enriched in CCV coats. This protein colocalizes with AP-2 at the plasma membrane by binding AP-2s α-ear domain, and interacts with AP-1, AP-2 and several GAE domain proteins termed GGA1, GGA2 and GGA3. NECAP2 is Involved in endocytosis.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
• Molecular Weight: 30.5 kDa (283 aa) confirmed by MALDI-TOF
• Purity: > 85% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MEESGYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA KGQMAYIKLE DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG RRAFIGIGFG DRGDAFDFNV ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL GFKEGQTIKL NIANMKKKEG AAGNPRVRPA STGGLSLLPP PPGGKTSTLI PPPGEQLAVG GSLVQPAVAP SSGGAPVPWP QPNPATADIW GDFTKSTGST SSQTQPGTGW VQF
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: NECAP2 NECAP endocytosis associated 2 [ Homo sapiens ]
• Synonyms: FLJ10420; NECAP2; NECAP endocytosis associated 2; adaptin ear-binding coat-associated protein 2; NECAP-2; OTTHUMP00000221695; OTTHUMP00000221697; OTTHUMP00000221698; NECAP endocytosis-associated protein 2
• Gene ID: 55707
• mRNA Refseq: NM_001145277
• Protein Refseq: NP_001138749
• MIM: 611624
• UniProt ID: Q9NVZ3
• Chromosome Location: 1p36.13