Recombinant Human NDUFAF2, His-tagged

• Cat.No.: NDUFAF2-1008H
• Product Overview: Recombinant human NDUFAF2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-169aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: NDUFAF2, also known as Mimitin, belongs to the complex I NDUFA12 subunit family. NADH dehydrogenase is an enzyme located in the inner mitochondrial membrane that catalyzes the transfer of electrons from NADH to coenzyme Q (CoQ). It is the "entry enzyme" of oxidative phosphorylation in the mitochondria. Mimitin protein acts as a molecular chaperone for mitochondrial complex I assembly.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 200mM NaCl
• Molecular Weight: 22kDa (189aa), confirmed by MALDI-TOF
• Purity: > 85% by SDS - PAGE
• Concentration: 0.5mg/ml (determined by Bradford assay)
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MGWSQDLFRA LWRSLSREVK EHVGTDQFGN KYYYIPQYKN WRGQTIREKR IVEAANKKEV DYEAGDIPTE WEAWIRRTRK TPPTMEEILK NEKHREEIKI KSQDFYEKEK LLSKETSEEL LPPPVQTQIK GHASAPYFGK EEPSVAPSST GKTFQPGSWM PRDGKSHNQ
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: NDUFAF2 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2 [ Homo sapiens ]
• Official Symbol: NDUFAF2
• Synonyms: NDUFAF2; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2; MMTN; B17.2L; mimitin; FLJ22398; NDUFA12L; mimitin, mitochondrial; OTTH- UMP00000161882; OTTHUMP00000221703; NDUFA12 -like protein; Myc-induced mitochondrial protein; B17.2-like; NDUFA12-like; NADH dehydrogenase [ubiqui- none] 1 alpha subcomplex assembly factor 2
• Gene ID: 91942
• mRNA Refseq: NM_174889
• Protein Refseq: NP_777549
• MIM: 609653
• UniProt ID: Q8N183
• Chromosome Location: 5q12.1
• Function: NADH dehydrogenase (ubiquinone) activity; electron carrier activity