Recombinant Human NDE1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: NDE1-6517H
• Product Overview: NDE1 MS Standard C13 and N15-labeled recombinant protein (NP_060138) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Mutations in this gene cause lissencephaly 4, a disorder characterized by lissencephaly, severe brain atrophy, microcephaly, and severe cognitive disability. Alternative splicing results in multiple transcript variants.
• Molecular Mass: 37.7 kDa
• AA Sequence: MEDSGKTFSSEEEEANYWKDLAMTYKQRAENTQEELREFQEGSREYEAELETQLQQIETRNRDLLSENNRLRMELETIKEKFEVQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRTPMPSSVEAERTDTAVQATGSVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYDQSPNRTGGPASGRSSKNRDGGERRPSSTSVPLGDKGLDTSCRWLSKSTTRSSSSCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NDE1 nudE neurodevelopment protein 1 [ Homo sapiens (human) ]
• Official Symbol: NDE1
• Synonyms: NDE1; nudE nuclear distribution E homolog 1 (A. nidulans); nudE nuclear distribution gene E homolog 1 (A. nidulans); nuclear distribution protein nudE homolog 1; FLJ20101; NUDE; nudE nuclear distribution gene E homolog 1; LIS1-interacting protein NUDE1, rat homolog; LIS4; NUDE1; HOM-TES-87
• Gene ID: 54820
• mRNA Refseq: NM_017668
• Protein Refseq: NP_060138
• MIM: 609449
• UniProt ID: Q9NXR1