Recombinant Human NCR1 protein, His-tagged

• Cat.No.: NCR1-897H
• Product Overview: Recombinant Human NCR1 protein(AAH64806.1)(Gln22-Asn254), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gln22-Asn254
• Tag: N-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human NKp46, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nkp46 Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: NCR1 natural cytotoxicity triggering receptor 1 [ Homo sapiens ]
• Official Symbol: NCR1
• Synonyms: NCR1; natural cytotoxicity triggering receptor 1; LY94, lymphocyte antigen 94 (mouse) homolog (activating NK receptor; NK p46); CD335; NK p46; NKP46; NK cell-activating receptor; natural killer cell p46-related protein; lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); LY94; NK-p46; FLJ99094
• Gene ID: 9437
• mRNA Refseq: NM_001145457
• Protein Refseq: NP_001138929
• MIM: 604530
• UniProt ID: O76036