Active Recombinant Cynomolgus NCR1 protein, lFc-tagged

• Cat.No.: NCR1-0521C
• Product Overview: Recombinant Cynomolgus NCR1 protein(Pro22-Arg257), fused with lFc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: lFc
• Protein Length: 22-257 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally Trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus NKp46, Llama IgG2b Fc Tag at 1 μg/mL (100 μL/well) can bind Anti-NKp46, hIgG4 with a linear range of 0.4-6 ng/mL. Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.
• Molecular Mass: This protein carries a llama IgG2b Fc tag at the C-terminus.The protein has a calculated MW of 54.7 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Pro22-Arg257
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.