Recombinant Human NARS2 Protein, His-tagged

• Cat.No.: NARS2-2477H
• Product Overview: Recombinant Human NARS2 Protein (Ile276-Leu477) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ile276-Leu477
• Description: This gene encodes a putative member of the class II family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of asparagine to tRNA molecules. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency 24 (COXPD24).
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 27 kDa
  Accurate Molecular Mass: 27 kDa
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: NARS2 asparaginyl-tRNA synthetase 2, mitochondrial (putative) [ Homo sapiens (human) ]
• Official Symbol: NARS2
• Synonyms: NARS2; asparaginyl-tRNA synthetase 2, mitochondrial (putative); probable asparagine--tRNA ligase, mitochondrial; asparagine tRNA ligase 2; mitochondrial (putative); FLJ23441; SLM5; asparagine--tRNA ligase; asparagine tRNA ligase 2, mitochondrial (putative); probable asparaginyl-tRNA synthetase, mitochondrial; asnRS
• Gene ID: 79731
• mRNA Refseq: NM_001243251
• Protein Refseq: NP_001230180
• MIM: 612803
• UniProt ID: Q96I59