Recombinant Human Myosin, Light Chain 4, Alkali; Atrial, Embryonic, His-tagged

• Cat.No.: MYL4-6857H
• Product Overview: Recombinant human MYL4 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 22.6 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: 1-197aa
• Description: MYL4 is a hexameric ATPase cellular motor protein. MYL4 is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL4 encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.15M NaCl.
• Molecular Mass: 22.6 kDa (205aa), confirmed by MALDI-TOF.
• AA Sequence: MAPKKPEPKK EAAKPAPAPA PAPAPAPAPA PEAPKEPAFD PKSVKIDFTA DQIEEFKEAF SLFDRTPTGE MKITYGQCGD VLRALGQNPT NAEVLRVLGK PKPEEMNVKM LDFETFLPIL QHISRNKEQG TYEDFVEGLR VFDKESNGTV MGAELRHVLA TLGEKMTEAE VEQLLAGQED ANGCINYEAF VKHIMSGLEH HHHHH
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MYL4 myosin, light chain 4, alkali; atrial, embryonic [ Homo sapiens ]
• Official Symbol: MYL4
• Synonyms: MYL4; myosin, light chain 4, alkali; atrial, embryonic; myosin, light polypeptide 4, alkali; atrial, embryonic; myosin light chain 4; ALC1; AMLC; GT1; myosin; atrial/fetal muscle; light chain; PRO1957; myosin light chain alkali GT-1 isoform; myosin, atrial/fetal muscle, light chain; myosin light chain 1, embryonic muscle/atrial isoform; myosin, light polypeptide 4, alkali; atrial, embryonic
• Gene ID: 4635
• mRNA Refseq: NM_001002841
• Protein Refseq: NP_001002841
• MIM: 160770
• UniProt ID: P12829
• Chromosome Location: 17q21-qter
• Pathway: Muscle contraction, organism-specific biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem; Striated Muscle Contraction, organism-specific biosystem; Striated Muscle Contraction, organism-specific biosystem
• Function: actin filament binding; actin monomer binding; calcium ion binding; myosin II heavy chain binding; structural constituent of muscle