Recombinant Human MYH1 protein, His-tagged

• Cat.No.: MYH1-7621H
• Product Overview: Recombinant Human MYH1 aa. (Tyr585~Ala794) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Tyr585~Ala794
• Description: Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.
• Form: Freeze-dried powder
• Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 9.6.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: MYH1 myosin heavy chain 1 [ Homo sapiens (human) ]
• Official Symbol: MYH1
• Synonyms: MYHa; HEL71; MYHSA1; MyHC-2x; MyHC-2X/D; epididymis luminal protein 71; myHC-IIx/d; myosin heavy chain 2x; myosin heavy chain IIx/d; myosin heavy chain, skeletal muscle, adult 1; myosin, heavy chain 1, skeletal muscle, adult; myosin, heavy polypeptide 1, skeletal muscle, adult; myosin-1
• Gene ID: 4619
• mRNA Refseq: NM_005963.3
• Protein Refseq: NP_005954.3
• UniProt ID: P12882