Recombinant Human myelin oligodendrocyte glycoprotein Protein, His tagged

• Cat.No.: MOG-041H
• Product Overview: The sequence (Accession # CAQ10087) corresponding to the extracellular domain of human MOG along with a 6×His tag was expressed in E. coli. The recombinant human MOG (H-rMOG) was purified from urea denatured bacterial lysate using immobilized metal affinity chromatography (IMAC).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-125aa
• Description: The product of this gene is a membrane protein expressed on the oligodendrocyte cell surface and the outermost surface of myelin sheaths. Due to this localization, it is a primary target antigen involved in immune-mediated demyelination. This protein may be involved in completion and maintenance of the myelin sheath and in cell-cell communication. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Tag: 6×His
• Form: Solution
• Molecular Mass: 14.2 kDa
• Bio-activity: Female C57BL/6 mice (8-10 weeks old) were immunized (s.c.) with 100 μg/animal of human rMOG in complete Freund's adjuvant followed by 400 ng/mouse of pertussis toxin on day 0 and day 2 (i.p.). Mice showed EAE symptoms such as limp tail, hind limb weakness, or hind limb paralysis after induction. DA rats immunized with 50 μg/animal of human rMOG at the base of the tail (s.c.) displayed EAE symptoms as well. Please note that no other EAE induction protocols were tested including IFA/cytokine model.
• Resuspension condition: Human MOG must be thoroughly mixed directly with Complete Freund's Adjuvant (CFA). Do not dilute recombinant human MOG with buffers that have pH greater than 4.5. Protein will precipitate at pH higher than 4.5.
• Endotoxin: Less than 0.1 EU per 1 μg of the protein as determined by Limulus Amebocyte Lysate (LAL) quantitative kinetic assay.
• Purity: ≥95% by SDS-PAGE
• Applications: ELISA; in vivo studies; Western Blotting
• Usage: Research use
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 1 mg/mL
• Storage Buffer: 25 mM sodium acetate buffer (pH=4.0)
• References: 1. Jayaram Bettadapura et.al. (1998) Journal of Neurochemistry 70 (4): 1593-1599
  2. Alfred R Oliver et al (2003) Journal of Immunology 171:462-468
  3. Hans-Christian Von Budingen et.al. (2001) Journal of Clinical Immunology 21 (3): 155-170
  4. Jerri-Anne Lyons et.al. (1999) European Journal of Immunology 29: 3432-3439
  5. Hans-Christian Von Budingen et.al. (2004) European Journal of Immunology 34: 2072-2083

Gene Information

• Gene Name: MOG myelin oligodendrocyte glycoprotein [ Homo sapiens (human) ]
• Official Symbol: MOG
• Synonyms: MOG; myelin oligodendrocyte glycoprotein; myelin-oligodendrocyte glycoprotein; MOG Ig-AluB; MOG alpha-5; MOGIG2; NRCLP7; MGC26137
• Gene ID: 4340
• mRNA Refseq: NM_001008228
• Protein Refseq: NP_001008229
• MIM: 159465
• UniProt ID: Q16653