Recombinant Human MRPL47 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: MRPL47-6443H
• Product Overview: MRPL47 MS Standard C13 and N15-labeled recombinant protein (NP_817125) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene is immediately adjacent to the gene for BAF complex 53 kDa subunit protein a (BAF53a), in a tail-to-tail orientation. Two transcript variants encoding different protein isoforms have been identified.
• Molecular Mass: 27.1 kDa
• AA Sequence: MAAAGLALLCRRVSSALKSSRSLITPQVPACTGLLHTTLSRKGLEEFFDDPKNWGQEKVKSGAAWTCQQLRNKSNEDLHKLWYVLLKERNMLLTLEQEAKRQRLPMPSPERLDKVVDSMDALDKVVQEREDALRLLQTGQERARPGAWRRDIFGRIIWHKFKQWVIPWHLNKRYNRKRFFALPYVDHFLRLEREKRARIKARKENLERKKAKILLKKFPHLAEAQKSSLVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: MRPL47 mitochondrial ribosomal protein L47 [ Homo sapiens (human) ]
• Official Symbol: MRPL47
• Synonyms: MRPL47; mitochondrial ribosomal protein L47; NCM1; L47mt; CGI-204; MRP-L47; 39S ribosomal protein L47, mitochondrial; mitochondrial large ribosomal subunit protein uL29m; nasopharyngeal carcinoma metastasis-related 1; nasopharyngeal carcinoma metastasis-related protein 1
• Gene ID: 57129
• mRNA Refseq: NM_177988
• Protein Refseq: NP_817125
• MIM: 611852
• UniProt ID: Q9HD33