Recombinant Human MPG, His-tagged

• Cat.No.: MPG-1009H
• Product Overview: Recombinant human MPG protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-298aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: MPG (DNA-3-methyladenine glycosylase) belongs to the DNA glycosylase MPG family. This protein initiates base excision repair in DNA by removing a wide variety of alkylated, deaminated, and lipid peroxidation-induced purine adducts.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 200mM NaCl, 1mM EDTA
• Molecular Weight: 33.9kDa (306aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• Purity: > 90% by SDS - PAGE
• Concentration: 0.25mg/ml (determined by Bradford assay)
• Sequences Of Amino Acids: MVTPALQMKK PKQFCRRMGQ KKQRPARAGQ PHSSSDAAQA PAEQPHSSSD AAQAPCPRER CLGPPTTPGP YRSIYFSSPK GHLTRLGLEF FDQPAVPLAR AFLGQVLVRR LPNGTELRGR IVETEAYLGP EDEAAHSRGG RQTPRNRGMF MKPGTLYVYI IYGMYFCMNI SSQGDGACVL LRALEPLEGL ETMRQLRSTL RKGTASRVLK DRELCSGPSK LCQALAINKS FDQRDLAQDE AVWLERGPLE PSEPAVVAAA RVGVGHAGEW ARKPLRFYVR GSPWVSVVDR VAEQDTQALE HHHHHH
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: MPG N-methylpurine-DNA glycosylase [ Homo sapiens ]
• Official Symbol: MPG
• Synonyms: MPG; N-methylpurine-DNA glycosylase; AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1; DNA-3-methyladenine glycosylase; OTTHUMP0000004- 5554; OTTHUMP00000067137; OTTHUMP00000194908; alkyladenine DNA glycosylase; 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; prolifera- tion-inducing protein 11; proliferation-inducing protein 16; N-methylpurine-DNA glycosylase, MPG; CRA36.1 (3-methyl-adenine DNA glycosylase); 3" end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p; EC 3.2.2.21; ANPG; MID1
• Gene ID: 4350
• mRNA Refseq: NM_002434
• Protein Refseq: NP_002425
• MIM: 156565
• UniProt ID: P29372
• Chromosome Location: 16p13.3
• Function: DNA N-glycosylase activity; alkylbase DNA N-glycosylase activity; damaged DNA binding; hydrolase activity; identical protein binding; protein binding