Recombinant Human/Mouse/Rat Smad3 protein, His&GST-tagged

• Cat.No.: Smad3-0256H
• Product Overview: Recombinant Human/Mouse/Rat Smad3 protein(NP_058049.3)(Met1-Ser425), fused with His and GST tag, was expressed in Insect Cells.

Specification

• Species: Human/Mouse/Rat
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-425 a.a.
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 2mM GSH, 10% glycerol, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The recombinant human / mouse / rat SMAD3 consists of 662 amino acids and has a calculated molecular mass of 75.9 kDa.
• Proteinlength: Met1-Ser425
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.