Recombinant Human MMP8 protein, His-tagged

• Cat.No.: MMP8-648H
• Product Overview: Recombinant Human MMP8 protein(P22894)(Phe21-Gly467), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Phe21-Gly467
• Form: Lyophilized from 0.22μm filtered solution in 50mM Tris,10mM CaCl2, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human MMP-8, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 1.93μg/ml determined by ELISA (QC Test).
• Molecular Mass: The protein has a predicted MW of 52.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: MMP8 matrix metallopeptidase 8 (neutrophil collagenase) [ Homo sapiens ]
• Official Symbol: MMP8
• Synonyms: MMP8; matrix metallopeptidase 8 (neutrophil collagenase); CLG1, matrix metalloproteinase 8 (neutrophil collagenase); neutrophil collagenase; PMNL collagenase; matrix metalloproteinase-8; matrix metalloproteinase 8 (neutrophil collagenase); HNC; CLG1; MMP-8; PMNL-CL
• Gene ID: 4317
• mRNA Refseq: NM_002424
• Protein Refseq: NP_002415
• MIM: 120355
• UniProt ID: P22894