Recombinant Human MMP1 protein, His-tagged

• Cat.No.: MMP1-26H
• Product Overview: Recombinant human MMP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: MMP1, also known as interstitial collagenase isoform 1, contains 4 hemopexin-like domains and is a member of the matrix metalloproteinase (MMP) family. It is capable of degrading a wide range of extracellular molecules and a number of bioactive molecules. It plays a central role in cell proliferation, migration, differentiation, angiogenesis, apoptosis and host defences.
• Form: Liquid. In 20mM MES buffer (pH 5.5) containing 10mM CaCl2, 100 mM NaCl, 0.05% Brij35, 30% glycerol.
• Molecular Mass: 53.1kDa (460aa)
  50-70kDa (SDS-PAGE under reducing conditions)
• AA Sequence: HSFPATLETQ EQDVDLVQKY LEKYYNLKND GRQVEKRRNS GPVVEKLKQM QEFFGLKVTG KPDAETLKVM KQPRCGVPDV AQFVLTEGNPRWEQTHLTYR IENYTPDLPR ADVDHAIEKA FQLWSNVTPL TFTKVSEGQA DIMISFVRGD HRDNSPFDGP GGNLAHAFQP GPGIGGDAHFDEDERWTNNF REYNLHRVAA HELGHSLGLS HSTDIGALMY PSYTFSGDVQ LAQDDIDGIQ AIYGRSQNPV QPIGPQTPKA CDSKLTFDAITTIRGEVMFF KDRFYMRTNP FYPEVELNFI SVFWPQLPNG LEAAYEFADR DEVRFFKGNK YWAVQGQNVL HGYPKDIYSS FGFPRTVKHI
  DAALSEENTG KTYFFVANKY WRYDEYKRSM DPGYPKMIAH DFPGIGHKVD AVFMKDGFFY FFHGTRQYKF DPKTKRILTL QKANSWFNCRKNLEHHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: MMP1 matrix metallopeptidase 1 (interstitial collagenase) [ Homo sapiens ]
• Official Symbol: MMP1
• Synonyms: MMP1; interstitial collagenase; fibroblast collagenase; matrix metalloprotease 1; matrix metalloproteinase 1; CLG; CLGN
• Gene ID: 4312
• mRNA Refseq: NM_001145938
• Protein Refseq: NP_001139410
• MIM: 120353
• UniProt ID: P03956
• Chromosome Location: 11q21-q22
• Pathway: Activation of Matrix Metalloproteinases, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Basigin interactions, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem
• Function: calcium ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding