Recombinant Human MLH1 Protein, His tagged

• Cat.No.: MLH1-13H
• Product Overview: Purified recombinant protein of Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1, Ser339-Glu558, with N-terminal His tag, expressed in E.coli.

Specification

• Species: Human
• Source: E. coli
• Tag: His
• Protein Length: 339-558 aa
• Description: The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC).
• Molecular Mass: 24.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Storage Buffer: 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 10% glycerol
• Concentration: >0.05 μg/μL as determined by microplate BCA method
• Shipping: Dry Ice

Gene Information

• Gene Name: MLH1 mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) [ Homo sapiens (human) ]
• Official Symbol: MLH1
• Synonyms: MLH1; mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli); COCA2, mutL (E. coli) homolog 1 (colon cancer, nonpolyposis type 2); DNA mismatch repair protein Mlh1; FCC2; HNPCC; HNPCC2; COCA2; hMLH1; MGC5172
• Gene ID: 4292
• mRNA Refseq: NM_000249
• Protein Refseq: NP_000240
• MIM: 120436
• UniProt ID: P40692