Recombinant Human Mitogen-Activated Protein Kinase Kinase Kinase 9, GST-Tagged

• Cat.No.: MAP3K9-1040H
• Product Overview: Recombinant human MAP3K9 (1-433) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Cat. No.: MAP3K9-1040H
• Description: MAP3K9 or Mixed-Lineage Kinase 1 is a mitogen-activated protein kinase kinase kinase capable of activating the c-Jun NH(2)-terminal kinase (JNK) pathway. The catalytic domain of MAP3K9 has amino acid sequence similarity to both the tyr-specific and the ser/thr-specific kinase classes. In addition, MAP3K9 contain 2 leu/ile-zipper motifs and a basic sequence near its C-termini. MAP3K9 is threonine (and possibly serine) phosphorylated at multiple sites in the activation loop, with phosphorylation of Thr312 required for full activation.
• Applications: Kinase Assay, Western Blot
• Molecular Weight: 77 kDa
• Expression System: Sf9 insect cells using baculovirus.
• Species: Human
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10mM glutathione, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
• Specific Activity: 116 nmol/min/mg
• Purity: > 85 %
• Concentration: 0.1 ug/ul
• Sequences: 1-433
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Insulin Signaling; p38 MAPK Signaling Pathway
• Protein length: 1-433 a.a.

Gene Information

• Gene Name: MAP3K9 mitogen-activated protein kinase kinase kinase 9 [ Homo sapiens ]
• Official Symbol: MAP3K9
• Synonyms: MAP3K9; MLK1; MEKK9; PRKE1; mitogen-activated protein kinase kinase kinase 9; EC 2.7.11.25; mixed lineage kinase 1 (tyr and ser/thr specificity); Mixed lineage kinase 1; EC 2.7.11
• Gene ID: 4293
• mRNA Refseq: NM_033141
• Protein Refseq: NP_149132
• MIM: 600136
• UniProt ID: P80192
• Chromosome Location: 14q24.3-q31
• Function: ATP binding; JUN kinase kinase kinase activity; MAP kinase kinase activity; nucleotide binding; protein homo- dimerization activity; protein serine/threonine kinase activity; protein tyrosine kinase activity