Recombinant Human Mitogen-Activated Protein Kinase-Activated Protein Kinase 3, His-tagged

• Cat.No.: MAPKAPK3-1268H
• Product Overview: Recombinant humanfull length MAPKAPK3, His-tagged.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: MAPKAPK3 encodes a member of the Ser/Thr proteinkinase family. This kinase functions as a mitogen-activated protein kinase(MAP kinase)- activated protein kinase. MAPKAPK3 are also known as extracellular signal-regulated kinases (ERKs), actas an integration point for multiple biochemical signals. This kinase wasshown to be activated by growth inducers and stress stimulation of cells. Invitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinasewere all able to phosphorylate and activate this kinase, which suggested therole of this kinase as an integrative element of signaling in both mitogenand stress responses. This kinase was reported to interact with,phosphorylate and repress the activity of E47, which is a basichelix-loop-helix transcription factor known to be involved in the regulationof tissue-specific gene expression and cell differentiation.
• Form: Liquid in 50 mMTris, pH 7.5 + 150 mM NaCl + 0.05 mM EDTA + 0.05% Triton X-100 + 2 mM DTT +50% glycerol
• Molecular Weight: 47.3 kDa
• Concentration: 0.42 mg/ml
• Purity: ≥ 90% as determinedby SDS-PAGE analysis
• Specific Activity: 660 nmole ofphosphate transferred to MAPKAPK2 substrate peptide (KKLNRTLSVA) per minuteper mg of total protein at 30°C. Activity determined at a final proteinconcentration of 1.67 µg/ml.
• Storage: Stable for 6 monthsin working aliquots at -80°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: MAPKAPK3
• Full Length: Full L.

Gene Information

• Gene Name: MAPKAPK3 mitogen-activatedprotein kinase-activated protein kinase 3 [ Homo sapiens ]
• Synonyms: MAPKAPK3;mitogen-activated protein kinase-activated protein kinase 3; 3PK; MAPKAP3;MAPKAPK-3; MAP kinase-activated protein kinase 3; MAPKAP kinase 3;OTTHUMP00000210805; OTTHUMP00000210806; OTTHUMP00000210807; chromosome 3pkinase; MAPK-activated protein kinase 3; EC 2.7.11.1; EC 2.7.11
• Gene ID: 7867
• mRNA Refseq: NM_004635
• Protein Refseq: NP_004626
• MIM: 602130
• UniProt ID: Q16644
• Chromosome Location: 3p21.3
• Pathway: Activated TLR4signalling; FAS pathway and Stress induction of HSP regulation; ImmuneSystem; Innate Immune System; MAP kinase activation in TLR cascade; MAPKsignaling pathway; MyD88 cascade initiated on plasma membrane; MyD88dependent cascade initiated on endosome; NFkB and MAP kinases activationmediated by TLR4 signaling repertoire; Signal Transduction; Signalling toERKs; TRAF6 Mediated Induction of proinflammatory cytokines; TRIF mediatedTLR3 signaling; Toll Like Receptor TLR6; VEGF signaling pathway; p38MAPKevents
• Function: ATP binding; MAPkinase kinase activity; nucleotide binding; protein binding; proteinserine/threonine kinase activity