Recombinant Human mitogen-activated protein kinase 15 Protein, His&Flag&StrepII tagged

• Cat.No.: MAPK15-129H
• Product Overview: Recombinant human MAPK15 protein (Q8TD08) (Met 1-Gly 357) with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus was expressed in E. coli.

Specification

• Description: Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking/secretion and genome integrity, in a kinase activity-dependent manner. Controls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation. Regulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling. Prevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar-coated proteins. Upon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion. Binds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA.
• Source: E. coli
• Species: Human
• Tag: N-Twin strep and C-His&Flag
• Protein length: 1-357aa
• Molecular Mass: 47.1 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: MAPK15 mitogen-activated protein kinase 15 [ Homo sapiens (human) ]
• Official Symbol: MAPK15
• Synonyms: MAPK15; mitogen-activated protein kinase 15; ERK7; ERK8; extracellular signal regulated kinase 8; ERK-7; ERK-8; MAPK 15; MAP kinase 15; extracellular regulated kinase 8 delta; extracellular signal-regulated kinase 7; extracellular signal-regulated kinase 8
• Gene ID: 225689
• mRNA Refseq: NM_139021
• Protein Refseq: NP_620590
• MIM: 618616
• UniProt ID: Q8TD08