Recombinant Human Mitogen-Activated Protein Kinase 14, His-tagged

• Cat.No.: MAPK14-1344H
• Product Overview: Recombinant human MAPK14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 43.7 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-360aa
• Description: MAPK14 is a member of the MAP kinase family. MAPK14 is most closely related to p38 MAP kinases(MAPKs). The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress-related transcription and cell cycle regulation, as well as in genotoxic stress response.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl.
• Molecular Mass: 43.7kDa (383aa), confirmed by MALDI-TOF.
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSQERPT FYRQELNKTI WEVPERYQNL SPVGSGAYGS VCAAFDTKTG LRVAVKKLSR PFQSIIHAKR TYRELRLLKH MKHENVIGLL DVFTPARSLE EFNDVYLVTH LMGADLNNIV KCQKLTDDHV QFLIYQILRG LKYIHSADII HRDLKPSNLA VNEDCELKIL DFGLARHTDD EMTGYVATRW YRAPEIMLNW MHYNQTVDIW SVGCIMAELL TGRTLFPGTD HIDQLKLILR LVGTPGAELL KKISSESARN YIQSLTQMPK MNFANVFIGA NPLAVDLLEK MLVLDSDKRI TAAQALAHAY FAQYHDPDDE PVADPYDQSF ESRDLLIDEW KSLTYDEVIS FVPPPLDQEE MES
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MAPK14 mitogen-activated protein kinase 14 [ Homo sapiens ]
• Official Symbol: MAPK14
• Synonyms: MAPK14; mitogen-activated protein kinase 14; CSBP1, CSBP2, CSPB1; Mxi2; p38; p38 MAP kinase; PRKM14; PRKM15; MAP kinase 14; p38alpha Exip; MAP kinase Mxi2; MAP kinase p38 alpha; CSAID-binding protein; Csaids binding protein; MAX-interacting protein 2; stress-activated protein kinase 2A; p38 mitogen activated protein kinase; mitogen-activated protein kinase p38 alpha; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug-binding protein; RK; CSBP; EXIP; CSBP1; CSBP2; CSPB1; SAPK2A; p38ALPHA
• Gene ID: 1432
• mRNA Refseq: NM_001315
• Protein Refseq: NP_001306
• MIM: 600289
• UniProt ID: Q16539
• Chromosome Location: 6p21.3-p21.2
• Pathway: ADP signalling through P2Y purinoceptor 1, organism-specific biosystem; ATF-2 transcription factor network, organism-specific biosystem; Activated TLR4 signalling, organism-specific biosystem; Activation of the AP-1 family of transcription factors, organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem
• Function: ATP binding; MAP kinase activity; MAP kinase kinase activity; NFAT2 protein binding; nucleotide binding; protein binding; protein serine/threonine kinase activity