Recombinant Human Mitogen-Activated Protein Kinase 11

• Cat.No.: GRN-4937H
• Product Overview: Recombinant human GRN is a 88.0 kDa homodimeric protein and s a single, glycosylated, polypeptide chain containing 593 amino acids.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: GRN is a single precursor protein of granulins which are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. Granulins are a variety of active, 6.0 kDa peptides and named granulin A (epithelin, granulin B (epithelin 2), granulin C, etc. Both the peptides and intact progranulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
• Biological Activity: The effects of phospho-ERK1/2 and non phospho-ERK1/2 by human progranulin-FLAG in THP-1 cells. To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37°C 0, 30, 60, min, respectively by adding the recombinant protein (100 ng/ml) to the THP-1 monocyte cell, which was maintained with serum starvation for 24 hrs. Recombinant proteins in lanes 1, 2, and 3 were subjected to THP-1 monocyte cell treatments over 0, 30, 60 mins, respectively.
• Molecular Weight: 88.0 kDa
• Form: Sterile filtered clear solution in PBS.
• Endotoxin Level: <0.1 ng/μg
• Appearance: Liquid
• Purity: ≥90% by SDS-PAGE and ≥90% by HPLC
• Storage: Progranulin although stable at 4°C for 3 weeks, should be stored at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Gene Information

• Gene Name: GRN granulin [ Homo sapiens ]
• Official Symbol: GRN
• Synonyms: GRN; granulin; GEP; GP88; PEPI; PGRN; PCDGF; granulins; acrogranin; progranulin; proepithelin; granulin-epithelin; PC cell-derived growth factor
• Gene ID: 2896
• mRNA Refseq: NM_002087
• Protein Refseq: NP_002078
• MIM: 138945
• UniProt ID: P28799
• Chromosome Location: 17q21.32
• Function: cytokine activity; growth factor activity