Recombinant Human MFI2 protein, His-tagged

• Cat.No.: MFI2-1692H
• Product Overview: Recombinant Human MFI2 protein(P08582-1)(Gly20-Cys709), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: C-His
• Protein Length: Gly20-Cys709
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD228, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 76.60 kDa. Due to glycosylation, the protein migrates to 78-88 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: MFI2 antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 [ Homo sapiens ]
• Official Symbol: MFI2
• Synonyms: MFI2; antigen p97 (melanoma associated) identified; melanotransferrin; CD228; FLJ38863; MAP97; membrane bound transferrin like protein; MGC4856; MTF1; membrane-bound transferrin-like protein; melanoma-associated antigen p97, isoform 2
• Gene ID: 4241
• mRNA Refseq: NM_005929
• Protein Refseq: NP_005920
• MIM: 155750
• UniProt ID: P08582