Recombinant Human METTL16 Protein, His-tagged

• Cat.No.: METTL16-032H
• Product Overview: Human recombinant C-terminal histidine-tagged human METTL16 protein (2-562) was purified from E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-562
• Description: RNA N6-methyltransferase that methylates adenosine residues at the N6 position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753, PubMed:30197299, PubMed:30197297). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed:28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed:28525753, PubMed:30197299, PubMed:30197297). Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing and/or stability of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression (PubMed:28525753). In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A (PubMed:28525753). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed:28525753, PubMed:29051200, PubMed:32266935). Also able to bind various lncRNAs, such as 7SK snRNA (7SK RNA) or 7SL RNA (PubMed:29051200). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed:27872311).
• Molecular Mass: 64.5 kDa
• Purity: ≥90% as determined by SDS-PAGE
• Stability: ≥ 6 months
• Storage: At -80 centigrade.
• Storage Buffer: 50 mM HEPES, pH 8.0, with 150 mM sodium chloride, 10% glycerol

Gene Information

• Gene Name: METTL16 methyltransferase 16, N6-methyladenosine [ Homo sapiens (human) ]
• Official Symbol: METTL16
• Synonyms: METTL16; methyltransferase 16, N6-methyladenosine; RNA N6-adenosine-methyltransferase METTL16; N6-adenosine-methyltransferase METTL16; U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase; U6 snRNA methyltransferase; methyltransferase 10 domain containing; methyltransferase 10 domain-containing protein; methyltransferase like 16; methyltransferase-like protein 16; putative methyltransferase METT10D; EC 2.1.1.346; EC 2.1.1.348
• Gene ID: 79066
• mRNA Refseq: NM_024086
• Protein Refseq: NP_076991
• UniProt ID: Q86W50