Recombinant Human Methionine adenosyltransferase I, alpha, His-tagged

• Cat.No.: MAT1A-1945H
• Product Overview: Recombinant human MAT1A protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques, 45.6 kDa (414aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: MAT1A catalyzes the formation of S-adenosyltransferase (AdoMet) for methionine catabolism in the liver. MAT1A expression also correlates with a differentiated phenotype, whereas liver cells expressing MAT2A present a dedifferentiated phenotype and lowered AdoMet synthesis. Likewise, NFκB and TNFα cause a switch from MAT1A to MAT2A expression in human hepatocellular carcinoma (HCC), which facilitates cancer cell growth.
• Purity: > 95% by SDS – PAGE.
• Physical Appearance: Liquid.
• Formulation: In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 0.1M NaCl, 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHS SGLVPRGSHM NGPVDGLCDH SLSEGVFMFT SESVGEGHPD KICDQISDAV LDAHLKQDPN AKVACETVCK TGMVLLCGEI TSMAMVDYQR VVRDTIKHIG YDDSAKGFDF KTCNVLVALE QQSPDIAQCV HLDRNEEDVG AGDQGLMFGY ATDETEECMP LTIILAHKLN ARMADLRRSG LLPWLRPDSK TQVTVQYMQD NGAVIPVRIH TIVISVQHNE DITLEEMRRA LKEQVIRAVV PAKYLDEDTV YHLQPSGRFV IGGPQGDAGV TGRKIIVDTY GGWGAHGGGA FSGKDYTKVD RSAAYAARWV AKSLVKAGLC RRVLVQVSYA IGVAEPLSIS IFTYGTSQKT ERELLDVVHK NFDLRPGVIV RDLDLKKPIY QKTACYGHFG RSEFPWEVPR KLVF.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Cysteine and methionine metabolism; Metabolic pathways; Selenoamino acid metabolism; Biological oxidations

Gene Information

• Gene Name: MAT1A methionine adenosyltransferase I, alpha [ Homo sapiens ]
• Synonyms: MAT1A; methionine adenosyltransferase I, alpha; Ams; MAT; SAMS; MATA1; SAMS1; AdoMet; AI046368; MAT 1; adoMet synthetase 1; methionine adenosyltransferase 1; S-adenosylmethionine synthetase isoform type-1; AdoMet synthetase; EC 2.5.1.6; MAT-I/III; AMS1; Methionine adenosyltransferase I/III
• Gene ID: 4143
• mRNA Refseq: NM_000429
• Protein Refseq: NP_000420
• MIM: 610550
• UniProt ID: Q00266
• Chromosome Location: 10q22
• Function: ATP binding; metal ion binding; methionine adenosyltransferase activity; nucleotide binding; transferase activity