Recombinant Human MEPE protein, His & T7-tagged

• Cat.No.: MEPE-5844H
• Product Overview: Recombinant Human MEPE aa. (Ser212~Asn445 (Accession # Q9NQ76)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.2kDa
• Protein length: Ser212~Asn445
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.2.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: MEPE matrix extracellular phosphoglycoprotein [ Homo sapiens (human) ]
• Official Symbol: MEPE
• Synonyms: OF45; matrix, extracellular phosphoglycoprotein with ASARM motif (bone); osteoblast/osteocyte factor 45; matrix extracellular phosphoglycoprotein
• Gene ID: 56955
• mRNA Refseq: NM_001184694.2
• Protein Refseq: NP_001171623.1
• UniProt ID: Q9NQ76