Recombinant Human ME3 Protein, His tagged

• Cat.No.: ME3-22H
• Product Overview: Recombinant Human ME3 Protein with His-tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
• Form: Liquid
• Molecular Mass: (Theoretical molecular weight) ~ 63.2 kDa
• AA Sequence: RWTPTAPAQGCHSKPGPARPVPLKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDKGHLATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVNPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVHGKAYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEATRKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGKTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDYAYKHNLASYYPEPKDKEAFVRSLVYTPDYDSFTLD
• Purity: > 90% as determined by SDS-PAGE
• Storage: Long term storage, please prepare aliquots and store at -20 to -80 centigrade. Avoid freeze/thaw cycles.
• Concentration: 0.1 mg/mL
• Storage Buffer: In 20 mM Tris-HCl, 1 M NaCl, pH 8.0, with 10% glycerol.

Gene Information

• Gene Name: ME3 malic enzyme 3, NADP(+)-dependent, mitochondrial [ Homo sapiens (human) ]
• Official Symbol: ME3
• Synonyms: ME3; malic enzyme 3, NADP(+)-dependent, mitochondrial; NADP-dependent malic enzyme, mitochondrial; malate dehydrogenase; pyruvic-malic carboxylase; malic enzyme, NADP+-dependent, mitochondrial; mitochondrial NADP(+)-dependent malic enzyme 3; NADP-ME; FLJ34862
• Gene ID: 10873
• mRNA Refseq: NM_001014811
• Protein Refseq: NP_001014811
• MIM: 604626
• UniProt ID: Q16798