Recombinant Human MCCC1 Protein, His-tagged

• Cat.No.: MCCC1-2429H
• Product Overview: Recombinant Human MCCC1 Protein (Ala512-Glu725) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ala512-Glu725
• Description: This gene encodes the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 27.4 kDa
  Accurate Molecular Mass: 29 kDa
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: MCCC1 methylcrotonoyl-CoA carboxylase 1 (alpha) [ Homo sapiens (human) ]
• Official Symbol: MCCC1
• Synonyms: MCCC1; methylcrotonoyl-CoA carboxylase 1 (alpha); methylcrotonoyl Coenzyme A carboxylase 1 (alpha); methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; MCCA; MCCase subunit alpha; 3-methylcrotonyl-CoA carboxylase 1; methylcrotonoyl-Coenzyme A carboxylase 1 (alpha); 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; 3-methylcrotonyl-CoA carboxylase biotin-containing subunit; MCC-B; FLJ25545; DKFZp686B20267
• Gene ID: 56922
• mRNA Refseq: NM_020166
• Protein Refseq: NP_064551
• MIM: 609010
• UniProt ID: Q96RQ3