Recombinant Human MCAM protein, His-tagged

• Cat.No.: MCAM-011H
• Product Overview: Recombinant Human MCAM protein(NP_006491.2)(Met1-Gly559), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Gly559
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant CD146 comprises 547 amino acids and predicts a molecular mass of 61.3 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 80-90 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MCAM melanoma cell adhesion molecule [ Homo sapiens ]
• Official Symbol: MCAM
• Synonyms: MCAM; melanoma cell adhesion molecule; cell surface glycoprotein MUC18; CD146; Gicerin; MUC18; melanoma adhesion molecule; cell surface glycoprotein P1H12; melanoma-associated antigen A32; melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen
• Gene ID: 4162
• mRNA Refseq: NM_006500
• Protein Refseq: NP_006491
• MIM: 155735
• UniProt ID: P43121